The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
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This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
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This video explains the fundamentals of ion exchange chromatography and demonstrates buffer selection for protein analysis. More information: https://www.agilent.com/en/products/liquid-chromatography/infinitylab-lc-workflow-solutions/infinitylab-bio-inert-lc-solutions/1260-infinity-ii-bio-inert-lc-system
Views: 61574 Agilent Technologies
Handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Views: 427233 GE Healthcare Life Sciences
http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 159332 Bio-Resource
Not all soils are created equal. Chemistry helps agriculture succeed in a variety of environments. This video explains how soils (often negatively charged) interact with nutrients (often positively charged) through cation exchange. The cation exchange capacity (CEC) of a soil determines how frequently and in what doses it should receive fertilizer. © 2016, NMSU Board of Regents. NMSU is an equal opportunity/affirmative action employer and educator. This material is based upon work supported by the Hispanic-Serving Institutions Education Grants Program, National Institute of Food and Agriculture, U.S. Department of Agriculture, under agreement No. 2014-38422-22089. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.
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This video demonstrates how to pour a DEAE-Sepharose column for the separation of the proteins haemoglobin and catalase. The video also shows the running of the column and the separation of the proteins.
Views: 16637 Nick Morris
ION EXCHANGE RESINS Ion exchange resins are cross lined polymer which contains some ions (Cations or Anions) which can be exchanged by the medium ions.
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Demineralized Water also known as Deionized Water, Water that has had its mineral ions removed. Mineral ions such as cations of sodium, calcium, iron, copper, etc and anions such as chloride, sulphate, nitrate, etc are common ions present in Water. Deionization is a physical process which uses specially-manufactured ion exchange resins which provides ion exchange site for the replacement of the mineral salts in Water with Water forming H+ and OH- ions. Ion exchange is an exchange of ions between two electrolytes or between an electrolyte solution and a complex. In most cases the term is used to denote the processes of purification, separation, and decontamination of aqueous and other ion-containing solutions with solid polymeric or mineralic "ion exchangers".
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This video explains the demineralisation process in detail. ion-exchange process. Water softening/water purification method. Removal of ions/minerals from hard water. Subscribe for more videos !!!.
Views: 11529 Revathi Purushothaman
Alkaline Anion Exchange Membrane Materials Development at Bae Group, Rensselaer Polytechnic Institute
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Dario Dekel, Technion Fuel Choices and Smart Mobility Summit 2017, Tel Aviv, Israel
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http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html High-Performance Anion-Exchange Chromatography (HPAE-PAD) enables faster and more accurate analysis or anionic analytes and carbohydrates without derivatization, including mono-, di-, tri-, oligo- and polysaccharides, sugar alcohols, and amino sugars. HPAE uses hydroxide-based eluents at high pH to produce anions from analytes that would not be anionic at neutral pH. Chromatography with these eluents is possible due the development of metal-free, polymer based chromatographic systems and pH stable (0 to 14) nonporous polymeric resins, in which small anion-exchange MicroBeads* carrying the anion-exchange functional groups are permanently attached electrostatically to a larger cation-exchange resin particle. The nonporous nature of the resin minimizes band-broadening and imparts highly effective separation of a wide variety of carbohydrates, including branched oligosaccharides. A range of different columns for carbohydrate separations from mono, di-, tri- to oligo- and polysaccharides is available under the Thermo Scientific Dionex CarboPac columns brand. http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html
Views: 1664 Thermo Scientific
This video is an explanation of column chromatography, we will speak about ion exchange chromatography, its princple and how to perform it
Views: 20305 Biomedical and Biological Sciences
Oligosaccharide profiling of biopharmaceutical and other glycoprotein products remains challenging for scientists who report that regardless of the technique used, more than one technique is often required for complete oligosaccharide identification. Recent collaborative studies have noted that while MALDITOF MS was faster, High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD), provided significantly better precision, allowing identification of structural isomers and simultaneous analysis of neutral and sialylated oligosaccharides.
Views: 2358 GENNews
This chromatography lecture explains about ion exchange chromatography.Web- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html This video tutorial explains the mechanisms of ion exchange chromatography and its use in biochemistry
Views: 68521 Shomu's Biology
An ion-exchange resin or ion-exchange polymer is an insoluble matrix normally in the form of small beads, usually white or yellowish, fabricated from an organic polymer substrate. The beads are typically porous, providing a high surface area. The trapping of ions occurs with the accompanying releasing of other ions; thus the process is called ion-exchange. There are multiple types of ion-exchange resin. Most commercial resins are made of polystyrene sulfonate. Ion-exchange resins are widely used in different separation, purification, and decontamination processes. The most common examples are water softening and water purification. In many cases ion-exchange resins were introduced in such processes as a more flexible alternative to the use of natural or artificial zeolites. Also, ion exchange resins are highly effective in the biodiesel filtration process. This video is targeted to blind users. Attribution: Article text available under CC-BY-SA Creative Commons image source in video
Views: 27907 Audiopedia
Electrolysers present the ideal solution to store excess energy. They give value to otherwise wasted energy and store it in hydrogen, an energy carrier that can store in nearly unlimited quantities, for unlimited time, to be used as fuel at any point in the future. Hydrogen from water electrolysis is clean and green. It is a solution for renewable variability and values excess energy supply. The AEM electrolyser from Enapter, used at the Phi Suea House is the most cost efficient on the market, and can handle intermittent renewable energy supply. For further details visit www.enapter.com
Views: 235 Mark Shiels
This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram This lecture explains about the instrumentation, principle of Ion exchange Chromatography. Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column—a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. Article source: wikipedia Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! Subscribe: https://www.youtube.com/channel/UCU91ilky-nR0VwKYFmJTsUA?sub_confirmation=1 HPLC Chromatography: https://youtu.be/9P_SIiMheZk UV Visible Spectroscopy: https://youtu.be/1mrUHrZ-0zk #BaaYo #Chromatography
Views: 31978 BaaYo
An breif introduction to cation exchange columns. 1. Draining the equilibration buffer. 0m0s 2. Load and run the Amino Acid (AA) mixture. 1m37s 2. Load Citrate buffer to run AA mixture further into column. 4m24s 3. Summary diagram? 6m56s Note the video was recorded during the class and includes a practical demonstration and a written explanation (pen and paper). These two discussions were spliced together and each cover the same material.
Views: 25687 Mark Temple
Allen lab vlog #131 7/14/2017 Wiki links on Nafion and the energy converter based on similar proton exchange membranes: https://en.wikipedia.org/wiki/Nafion https://en.wikipedia.org/wiki/Lonnie_Johnson_(inventor) https://en.wikipedia.org/wiki/Johnson_thermoelectric_energy_converter
Views: 1358 Peter Allen
For more information, visit http://www.bio-rad.com/yt/26/ngc. Shawn Anderson presents an introduction to ion exchange chromatography using Bio-Rad's chromatography instruments, columns, and media to demonstrate the technique. Shawn covers the basic principles of ion exchange chromatography and touches on applications for the separation of proteins and other biomolecules using a variety of anion and cation exchange media. Bio-Rad's Successful Chromatography Webinar series provides a great introduction to the different chromatography methods available for research applications. Most medium pressure chromatography columns work with Bio-Rad's NGC preparative chromatography system. The NGC is designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes the NGC systems the instruments of choice for method development and scale-up. NGC systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC system, a single hardware platform can be modified as needed to suit changing applications and scales. . http://www.bio-rad.com/evportal/destination/product?catID=MFCV7415&WT.mc_id=yt-pfd-ww-ngc-20130701-zytDmnoioJo We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 36939 Bio-Rad Laboratories
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Views: 564 KARBON AKTIF.ORG
What does Anion Exchange Resins mean in English?
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A detail information about what is aem sites. This content under the Creative Commons Attribution-ShareAlike License, all text used in this video is from wikipedia. I do not own it. I just make video out of this so that some people get some help.
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