This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 204847 Shomu's Biology
The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
Views: 18604 BioNetwork
This video explains the fundamentals of ion exchange chromatography and demonstrates buffer selection for protein analysis. More information: https://www.agilent.com/en/products/liquid-chromatography/infinitylab-lc-workflow-solutions/infinitylab-bio-inert-lc-solutions/1260-infinity-ii-bio-inert-lc-system
Views: 69112 Agilent Technologies
Handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Views: 441943 GE Healthcare Life Sciences
Not all soils are created equal. Chemistry helps agriculture succeed in a variety of environments. This video explains how soils (often negatively charged) interact with nutrients (often positively charged) through cation exchange. The cation exchange capacity (CEC) of a soil determines how frequently and in what doses it should receive fertilizer. © 2016, NMSU Board of Regents. NMSU is an equal opportunity/affirmative action employer and educator. This material is based upon work supported by the Hispanic-Serving Institutions Education Grants Program, National Institute of Food and Agriculture, U.S. Department of Agriculture, under agreement No. 2014-38422-22089. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.
Views: 104354 LearningGamesLab
ION EXCHANGE RESINS Ion exchange resins are cross lined polymer which contains some ions (Cations or Anions) which can be exchanged by the medium ions.
Views: 26213 vedupro
http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 166421 Bio-Resource
This video explains the demineralisation process in detail. ion-exchange process. Water softening/water purification method. Removal of ions/minerals from hard water. Subscribe for more videos !!!.
Views: 18157 Revathi Purushothaman
This video demonstrates how to pour a DEAE-Sepharose column for the separation of the proteins haemoglobin and catalase. The video also shows the running of the column and the separation of the proteins.
Views: 17248 Nick Morris
An ion-exchange resin or ion-exchange polymer is an insoluble matrix normally in the form of small beads, usually white or yellowish, fabricated from an organic polymer substrate. The beads are typically porous, providing a high surface area. The trapping of ions occurs with the accompanying releasing of other ions; thus the process is called ion-exchange. There are multiple types of ion-exchange resin. Most commercial resins are made of polystyrene sulfonate. Ion-exchange resins are widely used in different separation, purification, and decontamination processes. The most common examples are water softening and water purification. In many cases ion-exchange resins were introduced in such processes as a more flexible alternative to the use of natural or artificial zeolites. Also, ion exchange resins are highly effective in the biodiesel filtration process. This video is targeted to blind users. Attribution: Article text available under CC-BY-SA Creative Commons image source in video
Views: 29333 Audiopedia
This video is an explanation of column chromatography, we will speak about ion exchange chromatography, its princple and how to perform it
Views: 22881 Biomedical and Biological Sciences
Alkaline Anion Exchange Membrane Materials Development at Bae Group, Rensselaer Polytechnic Institute
Views: 858 Chulsung Bae
Engineering Chemistry (Analysis Of Water) Softening Process Follow me on Instagram:-https://www.instagram.com/ruchu305/ Facebook:-https://www.facebook.com/ruchu.singh.9
Views: 12231 Ruchi Upadhyay
Ion exchange chromatography in hindi - This lecture explains about the ion exchange chromatography in hindi. Ion exchange chromatography is used to separate proteins based on the or charge. Cation exchange chromatography as well as anion exchange chromatography are used to separate proteins from mixtures. Stay tuned to know more about the following - 1. What is ion exchange chromatography 2. Ion exchange chromatography principle 3. Ion exchange chromatography steps 4. Ion exchange chromatography mechanism. 5. Applications of ion exchange chromatography 6. Types of ion exchange chromatography 7. Difference between cation exchange chromatography and anion exchange chromatography For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the video lecture on ion exchange chromatography in hindi.
Views: 3265 Shomu's Biology
This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram This lecture explains about the instrumentation, principle of Ion exchange Chromatography. Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column—a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. Article source: wikipedia Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! Subscribe: https://www.youtube.com/channel/UCU91ilky-nR0VwKYFmJTsUA?sub_confirmation=1 HPLC Chromatography: https://youtu.be/9P_SIiMheZk UV Visible Spectroscopy: https://youtu.be/1mrUHrZ-0zk #BaaYo #Chromatography
Views: 50162 BaaYo
Electrolysers present the ideal solution to store excess energy. They give value to otherwise wasted energy and store it in hydrogen, an energy carrier that can store in nearly unlimited quantities, for unlimited time, to be used as fuel at any point in the future. Hydrogen from water electrolysis is clean and green. It is a solution for renewable variability and values excess energy supply. The AEM electrolyser from Enapter, used at the Phi Suea House is the most cost efficient on the market, and can handle intermittent renewable energy supply. For further details visit www.enapter.com
Views: 349 Mark Shiels
Demineralized Water also known as Deionized Water, Water that has had its mineral ions removed. Mineral ions such as cations of sodium, calcium, iron, copper, etc and anions such as chloride, sulphate, nitrate, etc are common ions present in Water. Deionization is a physical process which uses specially-manufactured ion exchange resins which provides ion exchange site for the replacement of the mineral salts in Water with Water forming H+ and OH- ions. Ion exchange is an exchange of ions between two electrolytes or between an electrolyte solution and a complex. In most cases the term is used to denote the processes of purification, separation, and decontamination of aqueous and other ion-containing solutions with solid polymeric or mineralic "ion exchangers".
Views: 750 Crescent Social
This chromatography lecture explains about ion exchange chromatography.Web- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html This video tutorial explains the mechanisms of ion exchange chromatography and its use in biochemistry
Views: 69549 Shomu's Biology
Oligosaccharide profiling of biopharmaceutical and other glycoprotein products remains challenging for scientists who report that regardless of the technique used, more than one technique is often required for complete oligosaccharide identification. Recent collaborative studies have noted that while MALDITOF MS was faster, High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD), provided significantly better precision, allowing identification of structural isomers and simultaneous analysis of neutral and sialylated oligosaccharides.
Views: 2498 GENNews
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations). Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances with a negative charge at higher pH levels. The tightness of the binding between the substance and the resin is based on the strength of the negative charge of the substance. https://en.wikipedia.org/wiki/Anion-exchange_chromatography Please support this channel and help me upload more videos. Become one of my Patreons at https://www.patreon.com/user?u=3823907
Views: 29 WikiTubia
Allen lab vlog #131 7/14/2017 Wiki links on Nafion and the energy converter based on similar proton exchange membranes: https://en.wikipedia.org/wiki/Nafion https://en.wikipedia.org/wiki/Lonnie_Johnson_(inventor) https://en.wikipedia.org/wiki/Johnson_thermoelectric_energy_converter
Views: 1526 Peter Allen
A detail information about what is aem sites. This content under the Creative Commons Attribution-ShareAlike License, all text used in this video is from wikipedia. I do not own it. I just make video out of this so that some people get some help.
Views: 104 InfoBox
http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html High-Performance Anion-Exchange Chromatography (HPAE-PAD) enables faster and more accurate analysis or anionic analytes and carbohydrates without derivatization, including mono-, di-, tri-, oligo- and polysaccharides, sugar alcohols, and amino sugars. HPAE uses hydroxide-based eluents at high pH to produce anions from analytes that would not be anionic at neutral pH. Chromatography with these eluents is possible due the development of metal-free, polymer based chromatographic systems and pH stable (0 to 14) nonporous polymeric resins, in which small anion-exchange MicroBeads* carrying the anion-exchange functional groups are permanently attached electrostatically to a larger cation-exchange resin particle. The nonporous nature of the resin minimizes band-broadening and imparts highly effective separation of a wide variety of carbohydrates, including branched oligosaccharides. A range of different columns for carbohydrate separations from mono, di-, tri- to oligo- and polysaccharides is available under the Thermo Scientific Dionex CarboPac columns brand. http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html
Views: 1744 Thermo Scientific
Cation Exchange Capacity = CEC CEC= measure of the soil’s ability to hold positively charged ions This influences the soil’s ability to hold onto essential nutrients and provides a buffer against soil acidification. Cation = Atom with a positive charge Anion = Atom with a negative charge Link to Lecture Slides: https://drive.google.com/open?id=1W1p-NLEpi23z-0mfeWz2ffE9z40h76NF *Full work cited for "Cation Exchange Capacity" can be viewed at... https://drive.google.com/open?id=1pM3D7fgbCzgKMFehgBhDxxGYM5uRUbt5
Views: 561 DeBacco University
For further reading about Ion Exchange Process, Please click on the link given below ... http://vedupro.blogspot.in/2013/05/ion-exchange-process-fundamentals-of.html ION EXCHANGE PROCESS -- DEIONIZATION PROCESS -- DEMINERALIZATION PROCESS Ion exchange resins are insoluble, cross-linked, long chain organic polymers with a micro porous structure, and the "functional groups" attached to the chains are responsible for the ion -- exchange properties. (-COOH, -SO3H etc) are capable of exchange their H plus ions with other cations, which comes in their contact, where as those containing basic functional with other cations, which comes in their contact, where as those containing their anions with other anions, which comes in their contact. The ion exchange resins may be classified as: (1). Cation Exchange Resins (2). Anion Exchange Resin
Views: 69074 vedupro
Botnání anexu plovoucího na vodní hladině. Perličky se mění z průhledných na mléčně bílé. Video v reálném čase. Swelling of anion exchanger floating on water surface. Beads are changing from transparent to white translucent/opaque. Real time
Views: 112 Ion Exchange & Power Engineering
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Views: 1018 Osmosia Water Treatment Machinery
DM Plant – De Mineralize, works on ion exchange process, we use ion exchange resins for this process. DM reduces electrical conductivity by removing minerals like calcium, magnesium, Sodium, potassium, chlorides, nitrates, sulphates and silica from water. There are three stages in DM Plant, Cation Column, Anion Column and Mixed Bed Column. Cation and Anion Columns are called Two Bed DM Plant. Mixed Bed Column is called Mixed Bed DM Plant. Two bed DM Plant brings down electrical conductivity to less than 30 micron Siemens and the ph would be in between 8 to 10. Mixed bed DM Plant works as polisher. The output water of Mixed is called pure water, it contains less than 1 micro Siemens electrical conductivity and ph will neutral (6.8 to 7.2). We have two companies in India, who manufactures ion exchange resins. One is Ion exchange India, other is Thermax. We manufacture, DM Plant in FRP Body and MSRL(Mild Steel Rubber Lined). We have portable DM Plants, and Industrial DM Plants. We provide Online Conductivity and Ph meters along with DM Plant to monitor water quality. Hydrochloric Acid (hcl) is used to regenerate cation ion exchange Resin. Sodium hydroxide (i.e Caustic soda flakes) used to regenerate anion exchange resin. For more information and purchase: Contact: email: [email protected] visit : www.tesconaquatech.com #dmplant #demineralizeplant #ionexchange
Views: 2409 Gillela Laxmikanth Reddy
De Kation-Anion wisselaar zorgt voor een grote hoeveelheid gedemineraliseerd aquariumwater. Gedemineraliseerd aquariumwater, of gedeioniseerd aquariumwater of kortweg demi-water kan gebruikt worden in het aquarium om de waterwaarden van het aquariumwater aan te passen. Vooral dan de KH (karbonnaathardheid of tijdelijke hardheid) en bijgevolg ook de PH (zuurtegraad) zullen lager worden alsook de GH (totale hardheid). Voor de mensen die geleidbaarheid van het aquariumwater meten: de geleidbaarheid zal ook dalen (microsiemens en ppm). Dit kan vooral handig zijn bij vissen die dit zachter water op prijs stellen. Naast de video over regenwater ( https://goo.gl/nDjASx ) en de video over omgekeerde osmose ( https://goo.gl/haCnbz ) kon de ionenwisselaar (kation-anion) natuurlijk niet ontbreken. In de video overloop ik de werking van de kation-anion-wisselaar, alsook het proces van regenereren met zoutzuur en natronloog (natriumhydroxyde) en toon ik ook beelden van de snelheid waarmee er demiwater wordt aangemaakt. **************************************************************** 👉Momenteel beschikbare t-shirts die je kan kopen:👈 👉Aquarium T-Shirts: https://goo.gl/tm14Lg 👈 **************************************************************** CONTACTEER MIJ VIA YOUTUBE https://youtu.be/addme/2hR_Xkxj0wp3W00RKgbH7RxU0Rzp7Q **************************************************************** Aquarium T-Shirts: https://goo.gl/tm14Lg Premium Aquarium Clubje: https://goo.gl/bstzNV ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Vergeet niet te abonneren om op de hoogte gehouden te worden van toekomstige video's, klik daarvoor op deze link: https://goo.gl/k5TjJ9 en klik vervolgens op het belletje die ernaast staat. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Facebook https://goo.gl/jB8ty4 Twitter https://goo.gl/pUJWNg Google+ https://goo.gl/4HygFA Pinterest https://goo.gl/RecXdk Youtube https://goo.gl/sxoZVZ Foursquare https://goo.gl/jzyUPi ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Af en toe maak ik in de beschrijving gebruik van affliliate links. Hou er dus rekening mee dat het zou kunnen dat je in bovenstaande beschrijving linken vindt naar affliliate websites.
Views: 1320 Aquarium Sunshine's Valley
Introduction to Ion Exchange Chromatography for Protein Purification.
Views: 10901 acr92651
Market Segment by Type, covers Strong Base Anion Resin Weak Base Anion Resin Market Segment by Applications, can be divided into Water Treatment Pharmaceutical Food Industry Other Browse the Complete Anion Exchange Resin Market Report with comprehensive Table Of Contents @ https://emarketorg.com/pro/global-%28north-america-europe-and-asia-pacific-south-america-middle-east-and-africa%29-anion-exchange-resin-market-2018-forecast-to-2023/
Views: 4 uttam sharma