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Ion exchange chromatography | cation exchange chromatography and anion exchange chromatography
 
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This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 151718 Shomu's Biology
Anion Exchange Chromatography (GFP Purification part 2 of 6)
 
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The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
Views: 16502 BioNetwork
Ion Exchange Chromatography
 
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Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/ion-exchange-chromatography Facebook link: https://www.facebook.com/aklectures Website link: http://www.aklectures.com
Views: 138197 AK LECTURES
Principles of Ion Exchange Chromatography
 
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All our Principles and Methodology handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Anion exchange chromatography
 
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Ion exchange chromatography is of two types: cation exchange and anion exchange. Anion exchange is when we have to separate anions from a mixture of cations and neutral ions. This video gives insight of anion exchange as a whole. Hope it helps!!!!
Views: 3060 BioKaryon
Ion Exchange Chromatography - Theory and Principle
 
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http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 147651 Bio-Resource
IonExchange
 
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How ion exchange can be used to soften hard water.
Views: 150178 Clayton Spencer
Types of Ion Exchange in Soils
 
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Types of ion exchange in soils : 1- between solid phase 2- between solid phase and plant root 3- between solid phase and soil solution 4- between soil solution and plant root
Views: 4127 Abdo Sherif
Ion Exchange Resins, Cation Exchange Resins, Anion Exchange Resins
 
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ION EXCHANGE RESINS Ion exchange resins are cross lined polymer which contains some ions (Cations or Anions) which can be exchanged by the medium ions.
Views: 21989 vedupro
The Principle Of Ion Exchange Chromatography, A Full Explanation
 
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This video is an explanation of column chromatography, we will speak about ion exchange chromatography, its princple and how to perform it
High-Performance Anion-Exchange Chromatography (HPAE-PAD)
 
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http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html High-Performance Anion-Exchange Chromatography (HPAE-PAD) enables faster and more accurate analysis or anionic analytes and carbohydrates without derivatization, including mono-, di-, tri-, oligo- and polysaccharides, sugar alcohols, and amino sugars. HPAE uses hydroxide-based eluents at high pH to produce anions from analytes that would not be anionic at neutral pH. Chromatography with these eluents is possible due the development of metal-free, polymer based chromatographic systems and pH stable (0 to 14) nonporous polymeric resins, in which small anion-exchange MicroBeads* carrying the anion-exchange functional groups are permanently attached electrostatically to a larger cation-exchange resin particle. The nonporous nature of the resin minimizes band-broadening and imparts highly effective separation of a wide variety of carbohydrates, including branched oligosaccharides. A range of different columns for carbohydrate separations from mono, di-, tri- to oligo- and polysaccharides is available under the Thermo Scientific Dionex CarboPac columns brand. http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html
Views: 1534 Thermo Scientific
Oligosaccharide Analysis: High-Performance Anion-Exchange Chromatography
 
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Oligosaccharide profiling of biopharmaceutical and other glycoprotein products remains challenging for scientists who report that regardless of the technique used, more than one technique is often required for complete oligosaccharide identification. Recent collaborative studies have noted that while MALDITOF MS was faster, High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD), provided significantly better precision, allowing identification of structural isomers and simultaneous analysis of neutral and sialylated oligosaccharides.
Views: 2163 GENNews
Cation Exchange
 
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Not all soils are created equal. Chemistry helps agriculture succeed in a variety of environments. This video explains how soils (often negatively charged) interact with nutrients (often positively charged) through cation exchange. The cation exchange capacity (CEC) of a soil determines how frequently and in what doses it should receive fertilizer. © 2016, NMSU Board of Regents. NMSU is an equal opportunity/affirmative action employer and educator. This material is based upon work supported by the Hispanic-Serving Institutions Education Grants Program, National Institute of Food and Agriculture, U.S. Department of Agriculture, under agreement No. 2014-38422-22089. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.
Views: 77068 LearningGamesLab
Anion Exchange Chromatography - separating haemoglobin and catalase
 
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This video demonstrates how to pour a DEAE-Sepharose column for the separation of the proteins haemoglobin and catalase. The video also shows the running of the column and the separation of the proteins.
Views: 15718 Nick Morris
Ion Exchange Chromatography | Principle, Instrumentation & Lab Experiment
 
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This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram This lecture explains about the instrumentation, principle of Ion exchange Chromatography. Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column—a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. Article source: wikipedia Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! Subscribe: https://www.youtube.com/channel/UCU91ilky-nR0VwKYFmJTsUA?sub_confirmation=1 HPLC Chromatography: https://youtu.be/9P_SIiMheZk UV Visible Spectroscopy: https://youtu.be/1mrUHrZ-0zk #BaaYo #Chromatography
Views: 5457 BaaYo
Ion Exchange Process, Fundamentals of Deionization by Ion Exchange, Demineralization Process
 
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For further reading about Ion Exchange Process, Please click on the link given below ... http://vedupro.blogspot.in/2013/05/ion-exchange-process-fundamentals-of.html ION EXCHANGE PROCESS -- DEIONIZATION PROCESS -- DEMINERALIZATION PROCESS Ion exchange resins are insoluble, cross-linked, long chain organic polymers with a micro porous structure, and the "functional groups" attached to the chains are responsible for the ion -- exchange properties. (-COOH, -SO3H etc) are capable of exchange their H plus ions with other cations, which comes in their contact, where as those containing basic functional with other cations, which comes in their contact, where as those containing their anions with other anions, which comes in their contact. The ion exchange resins may be classified as: (1). Cation Exchange Resins (2). Anion Exchange Resin
Views: 65397 vedupro
LEARN AND GROW !! ION EXCHANGE PROCESS FOR WATER SOFTENING !
 
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On this channel you can get education and knowledge for general issues and topics
Views: 112712 LEARN AND GROW
Alkaline Anion Exchange Membranes for Electrochemical Energy Conversion Technology
 
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Alkaline Anion Exchange Membrane Materials Development at Bae Group, Rensselaer Polytechnic Institute
Views: 518 Chulsung Bae
Introduction to Ion-exchange chromatography
 
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This video explains the fundamentals of ion exchange chromatography and demonstrates buffer selection for protein analysis. More information: https://www.agilent.com/en/products/liquid-chromatography/infinitylab-lc-workflow-solutions/infinitylab-bio-inert-lc-solutions/1260-infinity-ii-bio-inert-lc-system
Views: 52533 Agilent Technologies
Hydrogen Storage from Renewables - The Anion Exchange Membrane Electroliser
 
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Electrolysers present the ideal solution to store excess energy. They give value to otherwise wasted energy and store it in hydrogen, an energy carrier that can store in nearly unlimited quantities, for unlimited time, to be used as fuel at any point in the future. Hydrogen from water electrolysis is clean and green. It is a solution for renewable variability and values excess energy supply. The AEM electrolyser from Enapter, used at the Phi Suea House is the most cost efficient on the market, and can handle intermittent renewable energy supply. For further details visit www.enapter.com
Views: 85 Mark Shiels
Cation Exchange Chromatography
 
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Website: simunyubiomedvisuals.com
Views: 26444 Faith Simunyu
Cation Exchange Capacity (CEC)
 
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See http://soilweb.landfood.ubc.ca/labmodules/ Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Anions and Cation
 
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Description of anions and cations with sodium and chloride as examples.
Views: 13290 Andrew Wolf
Quick guide to performing ion exchange chromatography
 
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An breif introduction to cation exchange columns. 1. Draining the equilibration buffer. 0m0s 2. Load and run the Amino Acid (AA) mixture. 1m37s 2. Load Citrate buffer to run AA mixture further into column. 4m24s 3. Summary diagram? 6m56s Note the video was recorded during the class and includes a practical demonstration and a written explanation (pen and paper). These two discussions were spliced together and each cover the same material.
Views: 23909 Mark Temple
Ion-exchange resin
 
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An ion-exchange resin or ion-exchange polymer is an insoluble matrix normally in the form of small beads, usually white or yellowish, fabricated from an organic polymer substrate. The beads are typically porous, providing a high surface area. The trapping of ions occurs with the accompanying releasing of other ions; thus the process is called ion-exchange. There are multiple types of ion-exchange resin. Most commercial resins are made of polystyrene sulfonate. Ion-exchange resins are widely used in different separation, purification, and decontamination processes. The most common examples are water softening and water purification. In many cases ion-exchange resins were introduced in such processes as a more flexible alternative to the use of natural or artificial zeolites. Also, ion exchange resins are highly effective in the biodiesel filtration process. This video is targeted to blind users. Attribution: Article text available under CC-BY-SA Creative Commons image source in video
Views: 24566 Audiopedia
0821 4000 2080 Anion Exchange Resin Regeneration
 
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0821 4000 2080 Anion Exchange Resin Regeneration ADY WATER JUAL Resin Anion Kation http://www.karbonaktif.org/2013/05/jual-resin-lewatit.html Untuk info dan pemesanan hubungi : 022-7239019 0821 4000 2080 0856 2476 9005 0857 2352 9677 0813 2259 9149 Pin BB: 29d2de88 32dbbfb0 new pin : 5AC4B7116 fungsi resin kation dan anion, harga resin anion, harga resin kation, harga resin softener, jual resin kation, jual resin kation anion, manfaat resin softener, resin softener adalah,, water softener resin home depot, anion exchange resin example, anion exchange resin msds, anion exchange resin regeneration, anion resin exchange capacity,
Views: 488 KARBON AKTIF.ORG
Ion exchange chromatography
 
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This chromatography lecture explains about ion exchange chromatography.Web- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html This video tutorial explains the mechanisms of ion exchange chromatography and its use in biochemistry
Views: 66440 Shomu's Biology
Ion Exchange Process
 
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This animation shows the process that happens during ion exchange
Views: 19170 Alonso Rocha Tapia
Ion exchange chromatography
 
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For more information, log on to- http://shomusbiology.weebly.com/ This video lecture will explain the mechanism of ion exchange chromatography and the use of ion exchange chromatography in isolating charged molecules. Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html
Views: 17395 Shomu's Biology
Ion exchange
 
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The principle of ion exchange explained
Views: 9061 MetrohmTV
Ion Exchange resin cationic or anionic?
 
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ion exchange resin cationic or anionic?
Views: 1934 Prof. TI
Dario Dekel - Progress in the Critical Path of the Development of Anion Exchange Membrane Fuel Cells
 
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Dario Dekel, Technion Fuel Choices and Smart Mobility Summit 2017, Tel Aviv, Israel
Protein Purification Problems
 
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In this video I do a two-part problem involving an anion exchange column and a hydrophobic column.
Views: 6915 FortuneFavorsPrep
Ion Exchange Column
 
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This video demonstrates the use of a cation exchange column to convert a salt to an acid. This allows for the analysis of the total number of cations present in a given salt sample by titrating the resulting acid with a base
Views: 72959 crharrison
ion exchange chromatography
 
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Subject:Biochemistry Paper: Biochemcial techniques
Views: 3809 Vidya-mitra
022-Ion Exchange Chromatography
 
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Discussion of principles behind ion exchange chromatography
Part 30: Ion Exchange Chromatography
 
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Ion Exchange Chromatography, Cationic Exchanger, Anionic Exchanger, Stationary Phase in Ion Exchange Chromatography, Mobile Phase in Ion Exchange Chromatography, Solvent System in Ion Exchange Chromatography, CM- Cellulose, DEAE, TEAE
Views: 4039 Dr. Puspendra Classes
DEAE Sephacel Column:  Protein Purification via Ion Exchange
 
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Introduction to Ion Exchange Chromatography for Protein Purification.
Views: 8951 acr92651
Expert Insight - Linda Lopez - High-Performance Anion-Exchange Chromatography (HPAE-PAD)
 
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Excerpt of Linda Lopez talking about the High-Performance Anion-Exchange Chromatography (HPAE-PAD) which enables faster and more accurate analysis or anionic analytes and carbohydrates without derivatization, including mono-, di-, tri-, oligo- and polysaccharides, sugar alcohols, and amino sugars. Watch the complete interview at http://www.youtube.com/watch?v=zAUOkNr13NI Learn more at http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html
Views: 283 Thermo Scientific
Anion Exchange
 
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Replacing a Cl- with a BF4-
Views: 31 brenton gildner
Kation-Anion wisselaar voor aquarium : werking, regenereren, ...
 
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De Kation-Anion wisselaar zorgt voor een grote hoeveelheid gedemineraliseerd aquariumwater. Gedemineraliseerd aquariumwater, of gedeioniseerd aquariumwater of kortweg demi-water kan gebruikt worden in het aquarium om de waterwaarden van het aquariumwater aan te passen. Vooral dan de KH (karbonnaathardheid of tijdelijke hardheid) en bijgevolg ook de PH (zuurtegraad) zullen lager worden alsook de GH (totale hardheid). Voor de mensen die geleidbaarheid van het aquariumwater meten: de geleidbaarheid zal ook dalen (microsiemens en ppm). Dit kan vooral handig zijn bij vissen die dit zachter water op prijs stellen. Naast de video over regenwater ( https://goo.gl/nDjASx ) en de video over omgekeerde osmose ( https://goo.gl/haCnbz ) kon de ionenwisselaar (kation-anion) natuurlijk niet ontbreken. In de video overloop ik de werking van de kation-anion-wisselaar, alsook het proces van regenereren met zoutzuur en natronloog (natriumhydroxyde) en toon ik ook beelden van de snelheid waarmee er demiwater wordt aangemaakt. **************************************************************** 👉Momenteel beschikbare t-shirts die je kan kopen:👈 👉Aquarium T-Shirts: https://goo.gl/tm14Lg 👈 **************************************************************** CONTACTEER MIJ VIA YOUTUBE https://youtu.be/addme/2hR_Xkxj0wp3W00RKgbH7RxU0Rzp7Q **************************************************************** Aquarium T-Shirts: https://goo.gl/tm14Lg Premium Aquarium Clubje: https://goo.gl/bstzNV ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Vergeet niet te abonneren om op de hoogte gehouden te worden van toekomstige video's, klik daarvoor op deze link: https://goo.gl/k5TjJ9 en klik vervolgens op het belletje die ernaast staat. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Facebook https://goo.gl/jB8ty4 Twitter https://goo.gl/pUJWNg Google+ https://goo.gl/4HygFA Pinterest https://goo.gl/RecXdk Youtube https://goo.gl/sxoZVZ Foursquare https://goo.gl/jzyUPi ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Af en toe maak ik in de beschrijving gebruik van affliliate links. Hou er dus rekening mee dat het zou kunnen dat je in bovenstaande beschrijving linken vindt naar affliliate websites.
Ion Exchange Resins Know How
 
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The Video explane how to proper use ion exchange resins for laboratory tests. http://www.rfpconsulting.it/
Ion exchange resin Meaning
 
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Video is created with the help of wikipedia, if you are looking for accurate, professional translation services and efficient localization you can use Universal Translation Services https://www.universal-translation-services.com?ap_id=ViragGNG Video shows what ion exchange resin means. An insoluble matrix of small beads having a charged surface; used as an artificial zeolite in water softeners and in ion exchange chromatography.. Ion exchange resin Meaning. How to pronounce, definition audio dictionary. How to say ion exchange resin. Powered by MaryTTS, Wiktionary
Views: 2016 SDictionary

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